Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years
Keywords:
Sensitivity, Antibiotics, pattern of antibiotic susceptibility, pattern of microorganismsAbstract
Objective: To determine the prevalent bacteria causing wound infection and their trend of susceptibility to antibiotics over a period of last three years in flame burn cases. This is an observational descriptive study conducted at the Burns Center, Civil Hospital Karachi.
Methods: All available wound swabs culture and sensitivity reports of flame burn victims admitted during the period of January 2009 - December 2011 were included. Wound swab was usually taken from the burnt area that appears clinically deep, with pus or with thick eschar. In laboratory, microbial growth on any of the inoculated plates gram staining, biochemical identification and antibiotic sensitivity by disc diffusion method was done.
Statistical analysis was done through SPSS version 17.0 to determine the frequencies, mean ± SD and association of these culture and sensitivity of isolated organisms with antibiotics.
Result: Wound swab culture and sensitivity reports of 382 flame burnt patients were assessed. No significant relation was found between age, gender and duration of stay of a patient and bacterial colonization. Culture and sensitivity reports showing isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumonia, Proteus as major microorganisms. Pseudomonas Aeroginosa was found to be the most commonly isolated microorganism 36.6%(f=140). It was found to be highly sensitive with Imipenam throughout our period of study whereas Staphlococcus Aureus was found highly sensitive with vancomycin, doxicillin and Amoxicillin+ clauvilaunic acid (in sequence with their percentage sensitivity
in 2011).
Downloads
References
Abd-El-Aal AM, El-Hadidy ER, El-Mashad NB, ElSebaie AH. Antimicrobial effects of bee honey in comparison to antibiotics on organisms isolated from infected burns. Ann Burns Fire Disasters 2007;20:83-8.
Ahmad M, Hussain SS, Khan MI, Malik SA. Pattern of bacterial invasion in burn patients at the Pakistan Institute of Medical Sciences, Islamabad. Ann Burns Fire Disasters 2006;19:18-21.
Mehta M, Dutta P, Gupta V. Bacterial isolates from burn wound infections and their antibiograms. An eight year study. Indian J Plastic Surg 2007;40:25-8.
Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R et al. Emergence of new antibiotic resistant mechanism in India, Pakistan and the UK: a molecular, biological and epidemiological study. Lancet Infect Dis. 2010;10:597-602.
Marsh D, Sheikh A, Khalil A, Kamil S. Epidemiology of adults hospitalized with burns in Karachi, Pakistan Burns. 1996;22:225-9.
Bhat VG, Vasaikar SD. Bacteriologic profile and antibiogram of aerobic burn wound isolates in Mthatha, Eastern Cape, South Africa. South Afr J Epidemlol Infect 2010;25:16-9.
Al-Ibran E, Rao MH, Fatima K, Irfan S, Iqbal MS, Khan M. Current Bacteriological profile in Fire-burn victims and their associated mortality at the Burns Centre, Karachi-Pakistan. Pak J Med Sci 2011;27:789-92.
Oncul O, Ulkur E, Acar A, Turhan V, Yeniz E, Karacaer Z et al. Prospective analysis of nosocomial infections in a Burn Care Unit, Turkey. Indain J Med Res 2009;130:758-64.
Dhar S, Saraf R, Singh K, Raina B. Microbiological profile of chronic burn wounds among Patients admitted in Burn Unit. J K Sci 2007;9:182-5.
Srinivasan S, Vartak AM, Patil A, Saldanha J. Bacteriology of the burn wound at Bai Jerbai Wadia Hospital for children, Mumbai, India-A 13 year study, Part1- Bacteriological profile. Indian J Plastic Surg b2009;42:213-18.
Rajput A, Singh KP, Kumar V, Sexena R, Singh R. Antibacterial resistance pattern of aerobic bacteria isolates from burn patients in tertiary care hospital. Bio Med Res 2008;19:28-30.
Saha SK, Muazzam N, Begum SA, Chowdhury A, Islam MS, Parveen R. study on time-related changes in Aerobic bacterial Pattern of burn wound Infection. Faridpur Med Coll J 2011;6:41-5.
Ehmer-al-Ibran, Rao MH, Ali S, Raza A, Khanum A, Malik R. To compare the rate of mortality among fire burn inhalational and fire burn non inhalational injuries in admitted patients at burns centre civil hospital Karachi. Medi Forum Mont 2013; 24:64-6.
Rao MH, Khan S, Waseem T, Naeem S, Sabir S. Sepsis In Infants: Assessment Of Bacterial Pathogens and Their Antibiotic Susceptibility, A Study At Government Tertiary Care Hospital, Karachi. J Dow Uni Health Sci 2013;7:35-40.
Ehmer-Al-Ibran, Hassan M, Farooq Z, Rao MH. To evaluate the presence and spread of environmental pathogens and their sensitability. An epidemiological survey of the microbiological monitoring at burns ward Civil Hospital Karachi. Medi Forum 2013;24:74-8.
Singh NP, Goyal R, Manchanda V, Das S, Kaur I, Talwar V. Changing trends in bacteriology of burns in the burns unit, Delhi, India. Burns 2003;29:129-32.
Bayat M, Zia MA, Haghi M, Hemmatyar G, Toghyani M. Antibiotic resistance pattern of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa isolated from burnt patients in Urmia. Afr J Microbiol Res 2011;5:996-1000.
Yaseen M, Ahsan MM, Alam M. Changing Pattern of Bacterial Flora and antimicrobial resistance in patients with extensive burns. Surg 2008;18:22-6.
Bruesselaers N, Monstrey S, Snoeij T. Morbidity and Mortality of Bloodstream Infections in Patients With Severe. Am J Crit Care 2010;19:81-7.
Beheshti S, Zia M. Bacteriology of burns and antibiogram in an Iranianburn care center. Afr J Pharm Pharmacol 2011;5:538-41.
Imran M, Faheem M, Aslam V. Wound Infections and Culture Pattern In Pediatric Patients. J Postgrad Med Ins 2009;23:304-8.
Thyagarajan SP, Mathangi RK. Handbook of Burn Management. 2nd ed. Marella L, Hanumandass, Mathangr R, editors. Daryaganj, New Delhi: Jaypee Brothers; 1991.p.110-002
Snelling, C.F.T, Ronald AR, Waters WR. Comparison of Silver Sulpkadiazine and Gentamicin for topical prophylaxis against burn wound sepsis. Can Med Asso J 1978:119;466- 70.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Ehmer Al-Ibran, Sidra Meraj, Mahwish Nasim, Maria Khan, Masood Hussain Rao
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Articles published in the Journal of Dow University of Health Sciences are distributed under the terms of the Creative Commons Attribution Non-Commercial License https://creativecommons.org/ licenses/by-nc/4.0/. This license permits use, distribution and reproduction in any medium; provided the original work is properly cited and initial publication in this journal. 
