A HISTOLOGIC STUDY OF CARBIMAZOLE-INDUCED HYPERPLASIA OF ADENOHYPOPHYSIS WITH PROTECTIVE ROLE OF THYROXINE IN MALE ALBINO RATS

Abstract

O bjective: To assess the frequency and degree o f pituitary hyperplasia in albino rats made hypothyroid by Carbimazole, and the association o f severity of pituitary enlargement, and the response to treatment with throxine. To determine the microscopic changes occurring in Beta basophil cells (Thyroid stimulating hormone & Adreno corticotrophin hormone producing cells) o f Anterior Pituitary gland by giving an antithyroid drug, Carbimazole and Carbimazol plus Thyroxin on Anterior pituitary glands o f male albino rats with increasing time period.
Design Experimental study Place: Anatomy Department, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi.

Methods: Forty five healthy, young adult male albino rats were selected for the study. They were distributed into 3 main groups o f 15 rats each. Group A served as control while Group B received injection Carbimazole 6 ^gm/G body weight subcutaneously daily. Group-C were treated with injection Carbimazole 6 ^gm/G body weight subcutaneously plus injection Thyroxin 5 ^gm intraperitoneally daily for their respective period o f treatment. Each group was further subdivided into three sub-groups according to the period o f treatment they received i.e. 2, 4 & 6 weeks at the end o f which animals were sacrificed. The Pituitary glands were dissected out after processing and staining (Wilson-Ezrin method). The tissues were subjected to detailed
micrometric examination.