Prevalence of Bacteria in Drinking Water in Karachi andtheir Antimicrobial Susceptibility
Keywords:
Bacteria, drinking water, antibiotic resistance, antibiotic susceptibilityAbstract
Introduction:
In Pakistan, the bacteriological quality of drinking water is not closely monitored and prevalence of waterborne diseases due to the contamination of drinking water is among the most common problems facedi n urban and rural areas of Pakistan. The bacteria inhabiting these water sources carry genes which render them resistant to many antimicrobials. These genes can be transmitted to other non-resistant bacteria as well making the diseases caused by them hard to treat.
Methodology and Results:
We collected 100 drinking water collected from four water sources, namely boring water, tap water, filtered water and boiled water, randomly collected from different parts of Karachi by a sterile method. They were tested to determine the bacterial isolates present in them using the analytical profile indexing (API). The antimicrobial susceptibility profile of these bacteria was done using the Kirby Bauer disk diffusion method. It was estimated that Klebsiella is the most commonly found organism in the drinking water samples, followed by Pseudomonas and E.Coli, Staphylococcus aureus being the least common. The isolates were found to be most resistant to ampicillin (99%) and least resistant to amikacin (1%)while they were found to be most sensitive to amikacin (96%) and imipenem (96%).
Conclusion:
Better quality and standards of drinking water are necessary for healthy human life. Filtration and boiling of tap and boring water decreases the number of pathogens present in it making it better and healthier for human consumption.
Downloads
References
Farooqi A, Khan A, Kazmi SU. Investigation of a community outbreak of typhoid fever associated with drinking water. BMC Public Health 2009; 9:476.
Omezuruike OI, Damilola AO, Adeola OT, Fajobi EA, Olufunke SB. Microbiological and physiochemical analysis of different water samples used for domestic purposes in Abeokuta and Ojata, Lagos State, Nigeria. Afr J Biotechnol 2008; 7:617-21.
Amouei A, Miranzadeh MB, Shahandeh Z, Taheri T, Asharnia HA, Akbarpour S, et al. A study on microbial quality of drinking water in rural areas of Mazandaran province in north of Iran (2011). J Environ Protect 2012; 3:605-9.
Hannan A, Shan S, Arshad U. Bacteriological analysis of drinking water of 100 families of Lahore by filtration membrane technique and chromagar. Biomedica 2010; 26:152-6.
Anwar MS, Lateef S, Siddiqi GM. Bacteriological quality of drinking water in Lahore. Biomedica 2010; 26:66-9.
Aziz JA. Management of source and drinking-water quality in Pakistan. East Mediterr Health J 2005; 11: 1087-98.
Shar AH, Kazi YF, Zardari M, Soomro IH. Bacteriological quality of drinking water of Sukkur city. Pak J Med Res 2009; 48:88-90.
Kormas KA, Neofitou C, Pachiadaki M, Koufostathi E. Changes of the bacterial assemblages throughout an urban drinking water distribution system. Environ Monit Assess 2010; 165:27-38.
Rasheed F, Khan A, Kazmi SU. Bacteriological analysis, antimicrobial susceptibility and detection of 16s rRNA gene of Helicobacter Pylori by PCR in drinking water samples of earthquake affected areas and other parts of Pakistan. Malaysian J Microbiol 2009; 5:123-7.
Freeman MC, Trinies V, Boisson S, Mak G, Clasen T. Promoting house water treatment through Women’s self help groups in rural India: Assessing impact on drinking water quality and equity. Plos One 2012 ; 7:e44068.
Shar AH, Kazi YF, Soomro IH. Antibiotic susceptibility of thermo-tolerant Escherichia Coli 2 isolated from drinking water of Khairpur city, Sindh, Pakistan. Pak J Biol Sci 2009; 12:648-52.
Schwartz T, Kohmen W, Jansen B, Obst U. Detection of antibiotic-resistant bacteria and their resistance genes in waste water, drinking water and surface water biofilms. FEMS Microbiol Ecol 2003; 43:325-35.
Armstrong JL, Shigeno DS, Calomiris JJ, Seidler RJ. Antibiotic-resistant bacteria in drinking water. Appl Environ Microbiol 1981; 42:277-83.
Ochman H, Lawrence JG, Groisman EA. Lateral gene transfer and the nature of bacterial innovation. Nature 2000; 405:299-304.
Barlow M. What antimicrobial resistance has taught us about horizontal gene transfer. Methods Mol Biol 2009; 532:397-411.
Risebro HL, Breton L, Aird H, Hooper A, Hunter PR. Contaminated small drinking water supplies and risk of infectious intestinal disease: A Prospective Cohort Study. Plos One 2012; 7:e42762.
Kafia MS, Slaiman GM, Nazanin MS. Physical and chemical status of drinking water from water treatment plants on Greater Zab River. Sci Environ Manage 2009; 13:89-92.
Shar AH, Kazi YF, Kanhar NA, Soomro IH, Zia SM, Ghumro PB. Drinking water quality in Rohri City, Sindh, Pakistan. Afr J Biotechnol 2010; 9:7102-7.
Shar AH, Kazi YF, Kanhar NA, Soomro IH. Bacterial community patterns of municipal water of Sukkur city in different seasons. Afr J Biotechnol 2012; 11:2287-95.
Tallon P, Magajna B, Lofranco C, Leung KT. Microbial indicators of fecal contamination in water: A current perspective. Water Air Soil Poll 2005; 166:139-66.
Edberg SC, Rice EW, Karlin RJ, Allen MJ. Escherichia Coli: The best biological drinking water indicator for public health protection. J Appl Microbiol 2009; 29:106-16.
Spinks AT, Dunstan RH, Harrison T, Coombes P, Kuczera G. Thermal inactivation of water-borne pathogenic and indicator bacterias at sub-boiling temperatures. Water Res 2006; 40:1326-32.
Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas Aeruginosa: Our worst nightmare? Clin Infect Dis 2002; 34:634.
Colomer-Lluch M, Jofre J, Muniesa M. Antibiotic resistance genes in bacteriophage DNA Fraction of environmental samples. Plos One 2011; 6:e17549.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Sharmeen Amin
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Articles published in the Journal of Dow University of Health Sciences are distributed under the terms of the Creative Commons Attribution Non-Commercial License https://creativecommons.org/ licenses/by-nc/4.0/. This license permits use, distribution and reproduction in any medium; provided the original work is properly cited and initial publication in this journal.